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dnapk total  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc dnapk total
    DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, <t>CHK1,</t> <t>pDNApk</t> S2056, <t>DNApk</t> total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.
    Dnapk Total, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+dnapk/DNA-PKcs+Mouse+mAb/pmc08345136-78-77-80
    Average 95 stars, based on 87 article reviews
    dnapk total - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "The DNA Damage Response Is Differentially Involved in HPV-Positive and HPV-Negative Radioresistant Head and Neck Squamous Cell Carcinoma"

    Article Title: The DNA Damage Response Is Differentially Involved in HPV-Positive and HPV-Negative Radioresistant Head and Neck Squamous Cell Carcinoma

    Journal: Cancers

    doi: 10.3390/cancers13153717

    DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, CHK1, pDNApk S2056, DNApk total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.
    Figure Legend Snippet: DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, CHK1, pDNApk S2056, DNApk total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.

    Techniques Used: Inhibition, Clone Assay, Irradiation, Control, Western Blot, Molecular Weight

    Related Articles

    Immunofluorescence:

    Article Title: Caveolin-1 is Associated with Tumor Progression and Confers a Multi-Modality Resistance Phenotype in Pancreatic Cancer
    Article Snippet: Anti- cleaved caspase-9, cleaved PARP, phospho-Akt, total Akt, phospho-BRCA1, phospho-DNAPK, total BRCA1, total DNAPK, phospho-JAK2, total JAK2, phospho-STAT3, total STAT3, PIAS3, Src (Y416), Src (Y527), total Src, SOCS2, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK1/2, total ERK1/2, alpha tubulin, phospho-H2.AX, beta actin and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Androgen receptor as a mediator and biomarker of radioresistance in triple-negative breast cancer
    Article Snippet: The proteins were transferred to polyvinylidene difluoride membranes and probed using phospho-DNAPK (Abcam-CAT#124918), total DNAPK (Cell Signaling-CAT#12311), androgen receptor (Millipore-CAT#06-680) and GAPDH (Cell Signaling-CAT#2118L) antibodies followed by HRP-conjugated secondary antibodies (Sigma) then visualized using the enhanced chemiluminescence Western Blotting Detection Reagent.

    Article Title: MEK inhibitor GSK1120212-mediated radiosensitization of pancreatic cancer cells involves inhibition of DNA double-strand break repair pathways
    Article Snippet: Total ERK-1/2, phospho-ERK-1/2 (Thr202/Tyr204), phospho-BRCA1(Ser1524), phospho-DNAPK(Ser2056), total BRCA1, total DNAPK, phospho-H2AX, RAD51, 53BP1, RRM1,RRM2, phospho-Chk1(Ser345), phospho-Chk2(T68), phospho-p53(Ser15), phospho-ATR(Ser428), phospho-ATM(Ser1981) and β actin antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Staining:

    Article Title: Caveolin-1 is Associated with Tumor Progression and Confers a Multi-Modality Resistance Phenotype in Pancreatic Cancer
    Article Snippet: Anti- cleaved caspase-9, cleaved PARP, phospho-Akt, total Akt, phospho-BRCA1, phospho-DNAPK, total BRCA1, total DNAPK, phospho-JAK2, total JAK2, phospho-STAT3, total STAT3, PIAS3, Src (Y416), Src (Y527), total Src, SOCS2, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK1/2, total ERK1/2, alpha tubulin, phospho-H2.AX, beta actin and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Androgen receptor as a mediator and biomarker of radioresistance in triple-negative breast cancer
    Article Snippet: The proteins were transferred to polyvinylidene difluoride membranes and probed using phospho-DNAPK (Abcam-CAT#124918), total DNAPK (Cell Signaling-CAT#12311), androgen receptor (Millipore-CAT#06-680) and GAPDH (Cell Signaling-CAT#2118L) antibodies followed by HRP-conjugated secondary antibodies (Sigma) then visualized using the enhanced chemiluminescence Western Blotting Detection Reagent.

    Article Title: MEK inhibitor GSK1120212-mediated radiosensitization of pancreatic cancer cells involves inhibition of DNA double-strand break repair pathways
    Article Snippet: Total ERK-1/2, phospho-ERK-1/2 (Thr202/Tyr204), phospho-BRCA1(Ser1524), phospho-DNAPK(Ser2056), total BRCA1, total DNAPK, phospho-H2AX, RAD51, 53BP1, RRM1,RRM2, phospho-Chk1(Ser345), phospho-Chk2(T68), phospho-p53(Ser15), phospho-ATR(Ser428), phospho-ATM(Ser1981) and β actin antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Western Blot:

    Article Title: Caveolin-1 is Associated with Tumor Progression and Confers a Multi-Modality Resistance Phenotype in Pancreatic Cancer
    Article Snippet: Anti- cleaved caspase-9, cleaved PARP, phospho-Akt, total Akt, phospho-BRCA1, phospho-DNAPK, total BRCA1, total DNAPK, phospho-JAK2, total JAK2, phospho-STAT3, total STAT3, PIAS3, Src (Y416), Src (Y527), total Src, SOCS2, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK1/2, total ERK1/2, alpha tubulin, phospho-H2.AX, beta actin and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Androgen receptor as a mediator and biomarker of radioresistance in triple-negative breast cancer
    Article Snippet: The proteins were transferred to polyvinylidene difluoride membranes and probed using phospho-DNAPK (Abcam-CAT#124918), total DNAPK (Cell Signaling-CAT#12311), androgen receptor (Millipore-CAT#06-680) and GAPDH (Cell Signaling-CAT#2118L) antibodies followed by HRP-conjugated secondary antibodies (Sigma) then visualized using the enhanced chemiluminescence Western Blotting Detection Reagent.

    Article Title: MEK inhibitor GSK1120212-mediated radiosensitization of pancreatic cancer cells involves inhibition of DNA double-strand break repair pathways
    Article Snippet: Total ERK-1/2, phospho-ERK-1/2 (Thr202/Tyr204), phospho-BRCA1(Ser1524), phospho-DNAPK(Ser2056), total BRCA1, total DNAPK, phospho-H2AX, RAD51, 53BP1, RRM1,RRM2, phospho-Chk1(Ser345), phospho-Chk2(T68), phospho-p53(Ser15), phospho-ATR(Ser428), phospho-ATM(Ser1981) and β actin antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Expressing:

    Article Title: Caveolin-1 is Associated with Tumor Progression and Confers a Multi-Modality Resistance Phenotype in Pancreatic Cancer
    Article Snippet: Anti- cleaved caspase-9, cleaved PARP, phospho-Akt, total Akt, phospho-BRCA1, phospho-DNAPK, total BRCA1, total DNAPK, phospho-JAK2, total JAK2, phospho-STAT3, total STAT3, PIAS3, Src (Y416), Src (Y527), total Src, SOCS2, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK1/2, total ERK1/2, alpha tubulin, phospho-H2.AX, beta actin and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Androgen receptor as a mediator and biomarker of radioresistance in triple-negative breast cancer
    Article Snippet: The proteins were transferred to polyvinylidene difluoride membranes and probed using phospho-DNAPK (Abcam-CAT#124918), total DNAPK (Cell Signaling-CAT#12311), androgen receptor (Millipore-CAT#06-680) and GAPDH (Cell Signaling-CAT#2118L) antibodies followed by HRP-conjugated secondary antibodies (Sigma) then visualized using the enhanced chemiluminescence Western Blotting Detection Reagent.

    Article Title: MEK inhibitor GSK1120212-mediated radiosensitization of pancreatic cancer cells involves inhibition of DNA double-strand break repair pathways
    Article Snippet: Total ERK-1/2, phospho-ERK-1/2 (Thr202/Tyr204), phospho-BRCA1(Ser1524), phospho-DNAPK(Ser2056), total BRCA1, total DNAPK, phospho-H2AX, RAD51, 53BP1, RRM1,RRM2, phospho-Chk1(Ser345), phospho-Chk2(T68), phospho-p53(Ser15), phospho-ATR(Ser428), phospho-ATM(Ser1981) and β actin antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Activation Assay:

    Article Title: Caveolin-1 is Associated with Tumor Progression and Confers a Multi-Modality Resistance Phenotype in Pancreatic Cancer
    Article Snippet: Anti- cleaved caspase-9, cleaved PARP, phospho-Akt, total Akt, phospho-BRCA1, phospho-DNAPK, total BRCA1, total DNAPK, phospho-JAK2, total JAK2, phospho-STAT3, total STAT3, PIAS3, Src (Y416), Src (Y527), total Src, SOCS2, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK1/2, total ERK1/2, alpha tubulin, phospho-H2.AX, beta actin and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Androgen receptor as a mediator and biomarker of radioresistance in triple-negative breast cancer
    Article Snippet: The proteins were transferred to polyvinylidene difluoride membranes and probed using phospho-DNAPK (Abcam-CAT#124918), total DNAPK (Cell Signaling-CAT#12311), androgen receptor (Millipore-CAT#06-680) and GAPDH (Cell Signaling-CAT#2118L) antibodies followed by HRP-conjugated secondary antibodies (Sigma) then visualized using the enhanced chemiluminescence Western Blotting Detection Reagent.

    Article Title: MEK inhibitor GSK1120212-mediated radiosensitization of pancreatic cancer cells involves inhibition of DNA double-strand break repair pathways
    Article Snippet: Total ERK-1/2, phospho-ERK-1/2 (Thr202/Tyr204), phospho-BRCA1(Ser1524), phospho-DNAPK(Ser2056), total BRCA1, total DNAPK, phospho-H2AX, RAD51, 53BP1, RRM1,RRM2, phospho-Chk1(Ser345), phospho-Chk2(T68), phospho-p53(Ser15), phospho-ATR(Ser428), phospho-ATM(Ser1981) and β actin antibodies were purchased from Cell Signaling Technology (Danvers, MA).



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    dnapk total  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc dnapk total
    DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, <t>CHK1,</t> <t>pDNApk</t> S2056, <t>DNApk</t> total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.
    Dnapk Total, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+dnapk/DNA-PKcs+Mouse+mAb/pmc08345136-78-77-80
    Average 95 stars, based on 1 article reviews
    dnapk total - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    total dnapk  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc total dnapk
    DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, <t>CHK1,</t> <t>pDNApk</t> S2056, <t>DNApk</t> total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.
    Total Dnapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+dnapk/DNA-PKcs+Mouse+mAb/pmc05562815-169-13-15
    Average 95 stars, based on 1 article reviews
    total dnapk - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, CHK1, pDNApk S2056, DNApk total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.

    Journal: Cancers

    Article Title: The DNA Damage Response Is Differentially Involved in HPV-Positive and HPV-Negative Radioresistant Head and Neck Squamous Cell Carcinoma

    doi: 10.3390/cancers13153717

    Figure Lengend Snippet: DDR inhibition in combination with RT radiosensitized HPV-positive and HPV-negative radioresistant HNSCC. ( A ) HPV-negative and HPV-positive radioresistant clones were pretreated for 2 h with DMSO (0 µM) or with indicated concentrations of DDR inhibitors and irradiated with the indicated doses. Medium was refreshed 24 h after drug exposure. Relative clonogenic survival was determined and is shown as the mean ± SEM clonogenic survival fraction of RT treated conditions relative to vehicle treated control cells, n = 3. ( B ) HPV-negative and HPV-positive radioresistant clones were treated with DMSO (−) or with indicated concentrations of DDR inhibitors. Cell lysates were prepared after 2 and 24 h of drug exposure. Immunoblotting of the following proteins was performed: pCHK1 S296, CHK1, pDNApk S2056, DNApk total, PAR, Vinculin and Beta-actin. KDA is the molecular weight as determined by the protein standard.

    Article Snippet: The following antibodies were used: pATM S1981 (13050S CST, Danvers, MA, USA), ATM (2873S CST, Danvers, MA, USA), pATR S428 (28538 CST, Danvers, MA, USA), ATR (27903 CST, Danvers, MA, USA), pCHK2 T68 (2197 CST, Danvers, MA, USA), CHK2 (3440 CST, Danvers, MA, USA), pCHK1 S345 (2348 CST, Danvers, MA, USA), pCHK1 S296 (2349 CST, Danvers, MA, USA), CHK1 (2360 CST, Danvers, MA, USA), pH3 S10 (53348 CST, Danvers, MA, USA), pDNApk S2056 (Abcam, Cambridge, MA, USA), DNApk total (12311S CST, Danvers, MA, USA), PAR (Enzo Life Sciences, Farmingdale, NY, USA), Vinculin (Sigma-Aldrich, Saint-Louis, MO, USA) and beta-actin (CST, Danvers, MA, USA).

    Techniques: Inhibition, Clone Assay, Irradiation, Control, Western Blot, Molecular Weight